Research Paper Volume 12, Issue 13 pp 12812—12840

PHLPP2 is regulated by competing endogenous RNA network in pathogenesis of colon cancer

Figure 9. Overexpression of LINC00402, LINC00461, and SFTA1P enhances the inhibition of PHLPP2 on HT-29 cell proliferation, migration and invasion, and promotion on cell apoptosis. HT-29 cells were transfected with NC, PHLPP2, PHLPP + pcDNA-LINC00402, PHLPP + pcDNA-LINC00461 or PHLPP + pcDNA-SFTA1P. (A) Fluorescence image (200 ×) by EdU labeling showed cell proliferation. (B) Flow cytometry detected cell apoptosis. (C) Scratch test (100 ×) revealed cell migration. (D) Transwell assay (100 ×) suggested cell invasion. (E) Western blot analysis of EMT-related proteins (E-cadherin, N-cadherin, and Vimentin), apoptosis-related proteins (cleaved Caspase-3 and Bax) and Akt3-p27 pathway-related proteins (Akt1, Akt3, and p27) in HT-29 cells normalized to GAPDH. * p < 0.05 vs. the NC group; # p < 0.05 vs. the PHLPP2 group. Measurement data in this Figure were expressed as the mean ± standard deviation, with comparisons among multiple groups conducted by One-Way ANOVA; the experiment was repeated three times independently.