Research Paper Volume 12, Issue 19 pp 18970—18981

LINC00963 facilitates acute myeloid leukemia development by modulating miR-608/MMP-15

Figure 4. Overexpression of LINC00963 promoted AML cell growth and EMT progression. (A) The expression of LINC00963 was measured by RT-qPCR in both THP-1 and HL-60 AML cells. (B) CCK-8 assay showed that overexpression of LINC00963 accelerated cell growth in both THP-1 and HL-60 cells. (C) Elevated expression of LINC00963 facilitated ki-67 expression in both THP-1 and HL-60 cells. (D) RT-qPCR assay indicated that elevated expression of LINC00963 accelerated PCNA levels in both THP-1 and HL-60 cells. (E) Ectopic expression of LINC00963 enhanced Vimentin, N-cadherin and ZEB1 expression and decreased E-cadherin expression in both THP-1 and HL-60 cells. *p<0.05, **p<0.01 and ***p<0.001. GAPDH was used as the internal control.