Figure 3. Down-regulation of TopBP1 significantly suppressed the proliferation of both 22RV1 (A) and LNCaP (B, C) qRT-PCR was performed to detect alteration of the TopBP1 expression. (D) Western blotting was performed to detect alteration of TopBP1. (E–H) Down-regulation of TopBP1 suppresses the migration of prostate cancer. (E, G) Represented images of two separated experiments in each cell line are showed. The data presented are mean ± SD for three independent experiments. **P<0.01 compared with NC, ***P<0.001 compared with NC.