Figure 4. CUEDC1 regulates TβRI/Smad signaling pathway. (A, B) Western blotting analysis of components of the TβR I/Smad signaling pathway and Snail in NSCLC cells. (C) The migration and invasion ability of shRNA/CUEDC1 or negative control in H1299 and A549 cells, with or without SB431542 treatment, was detected using transwell assays. P values were calculated using Student’s t-test. (D) H1299 and A549 cells stably transfected with CUEDC1-shRNA or empty vector were treated with 10 μM SB431542 for 24 h, and EMT markers was determined by immunoblotting. (E) CUEDC1-knockdown A549 cells or negative control A549 cells were treated with or without SB431542 for 24 h. Cells were immunostained with the indicated antibodies against EMT marker proteins. Experiments were performed at least three times. The data are expressed as the mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001.