Research Paper Volume 12, Issue 20 pp 20047—20068

CUEDC1 inhibits epithelial-mesenchymal transition via the TβRI/Smad signaling pathway and suppresses tumor progression in non-small cell lung cancer

Figure 6. CUEDC1 inhibited the metastasis of NSCLC in vivo. (A) A total of 1×106 of CUEDC1-knockdown or control cells were injected into tail vein of nude mice. After injection, bioluminescence images were monitored at the indicated time points. The number of metastatic sites is shown as the mean ± SEM; n=5 mice per group, P values were calculated using Student’s t-test. (B) The lungs were removed eight weeks later. Bioluminescence images and representative lung images of lung metastatic nodules are shown distinctly, and the numbers of metastatic nodules were measured. The data were statistically analysed by t-test and show the mean ± SEM. (C) Microscopic images of lung tissue sections stained by hematoxylin and eosin. The number of nodules on the lungs of nude mice was quantified (n=5 per group). Statistical analysis was performed using Student’s t-test. (D) CUEDC1, Smurf2, TβRI and E-cadherin expression were tested by immunohistochemical staining in lung tissues of mice (magnification, ×100 and ×400). The data are expressed as the mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001.