Research Paper Volume 12, Issue 20 pp 20047—20068

CUEDC1 inhibits epithelial-mesenchymal transition via the TβRI/Smad signaling pathway and suppresses tumor progression in non-small cell lung cancer

Figure 8. CUEDC1 knockdown promotes cell proliferation in vivo. (A) A total of 1 × 107 of CUEDC1-knockdown A549 cells and negative control A549 cells were injected into the left and right flanks of female nude mice (n=8). After injection, bioluminescence images were monitored at the indicated time points. (B) Tumor growth was monitored every 5 days after implantation. The data are shown as the mean ± SEM (two-tailed Student’s t-test). (C) Images of the tumor lumps from the indicated groups at the endpoint of the experiment. (D, E) The volume (D) and weight (E) of the tumors excised are the mean ± SEM. Statistical analysis was calculated using Student’s t-test. (F) IHC analysis of CyclinD1 and Bcl-2 expression. (G) A significant negative correlation was observed between CUEDC1 and CyclinD1, Bcl-2 in mice tumors. Statistical analysis was performed using χ2 test. (H) TUNEL detection showed correlation between CUEDC1 expression and apoptosis level. The data are expressed as the mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001.