Priority Research Paper Volume 12, Issue 11 pp 10041—10058

Lactate dehydrogenase expression modulates longevity and neurodegeneration in Drosophila melanogaster

Figure 6. Neuronal knockdown of Ldh delays age-related neurodegeneration. (A) Ldh mRNA levels were significantly reduced in the heads of 55-day old elavts>LdhRNAi compared to age-matched elavts>RNAicontrol flies (n=3; Unpaired t-test with Welch’s correction *p<0.05). (B) Survival curves of elavts>LdhRNAi (n=187) and elavts>RNAicontrol (n=195). Median lifespan was significantly extended in elavts>LdhRNAi flies compared to control (Gehan-Breslow-Wilcoxon test; p<0.0001; see also Supplementary Table 3). (C, D) Representative brain section images of 75-day old (C) elavts>RNAicontrol and (D) elavts>LdhRNAi flies (arrowheads indicate vacuoles). (E, F) Graphs showing that the average area (E) and number (F) of vacuoles were significantly lower in the brains of 75-day old elavts>LdhRNAi flies (n=48) compared to controls (n=46). Statistical significance determined by unpaired t-test with Welch’s correction *p<0.05. Bars indicated SEM.