Priority Research Paper Volume 12, Issue 10 pp 8766—8789

Muscle-dependent regulation of adipose tissue function in long-lived growth hormone-mutant mice

Figure 8. Adipose tissue macrophage infiltration and macrophage M1-M2 polarization of tissue-specific GHR KO mice (LKO, MKO and FKO). The three left panels show relative protein expression (Arginase1 and iNOS) in brown fat, inguinal and perigonadal adipose tissues of 24-week-old LKO (A), MKO (B), and FKO (C) was normalized to β-actin levels. Values are mean ±SEM (n = 4). *P < 0.05 versus WT. M = males; F = females. The three right panels show quantitative RT-PCR analysis of total RNA isolated from brown fat, inguinal and perigonadal adipose tissues of 24-week-old LKO (A), MKO (B), and FKO (C) mice and WT mice for IL-6, TNFα, MCP-1 mRNAs. Data (mean ± SEM; n = 4) were normalized by the amount of GAPDH mRNA and expressed relative to the corresponding male WT value. *P < 0.05 versus WT.