Research Paper Volume 12, Issue 13 pp 13005—13022

MicroRNA-124 inhibits macrophage cell apoptosis via targeting p38/MAPK signaling pathway in atherosclerosis development

Figure 4. Effects of miR-124 on pro-inflammatory cytokines and anti-inflammatory cytokines in vitro and in vivo. (A) ELISA assay analysis of the protein levels of pro-inflammatory cytokines in RAW264.7 macrophage cells. (B) ELISA assay analysis of the protein levels of anti-inflammatory cytokines in RAW264.7 macrophage cells. RAW264.7 cells were transfected with the miR-124 mimics, inhibitor or negative control, then treated with ox-LDL. (C) ELISA analysis of the protein levels of pro-inflammatory cytokines in blood serum of atherosclerosis model mice treated with agomir control or agomir-124. (D) ELISA analysis of the protein levels of anti-inflammatory cytokines. NC, negative control; iNC, negative control of miR-124 inhibitor. **, P <0.01, ***, P <0.001 compared with control group. ## P < 0.01 compared with iNC group.