Research Paper Volume 12, Issue 13 pp 13005—13022

MicroRNA-124 inhibits macrophage cell apoptosis via targeting p38/MAPK signaling pathway in atherosclerosis development

Figure 6. miR-124 suppressed the expression of pro-apoptotic factors but promoted the expression of anti-apoptotic factors. (A) Immunohistochemistry analysis of the pro-apoptotic factors and anti-apoptotic factors. Bar = 100 μm. (B) Western blot analysis of the protein levels of the pro-apoptotic factors and anti-apoptotic factors in RAW264.7 cells transfected with miR-124 mimics, inhibitor, negative control or miR-124 mimics + p38 pcDNA, then treated with ox-LDL. NC, negative control of miR-124 mimics; iNC, negative control of miR-124 inhibitor. β-actin was used as an internal reference in Western blot. *, P < 0.05, **, P < 0.01, ***, P < 0.001 compared with control group.