Figure 1. Increased thermogenic activity and improved physical performance in LLE mice. (A) Kaplan-Meier survival curve for long-term low-dose (3.5% v/v) ethanol intake (LLE) mice and untreated wild-type (WT) mice. (n=60 for each group). (B–D) Daily food intake, daily liquid intake, and daily calorie intake of 32-week-old LLE and WT mice (n=25 for each group). (E) Change in body weight (n= for each group). (F) X-axis movement of 44-week-old LLE and WT mice, an indicator of spontaneous locomotor activity in CLAMS system (n=8 for each group). (G–J) Oxygen consumption rate, CO2 production rate, energy expenditure rate, and respiratory exchange ratio (RER) of 44-week-old LLE and WT mice (n=8 for each group). (K–L) Distance run on treadmill and time to fall from an accelerating rotarod measured at 8, 12, 18 months, (n=10 for each group) Data are presented as mean ± SEM. *, P<0.05, **, P< 0.01, ***, P<0.001.