Figure 1. Confocal images of microglia and astrocytes in the post-ischemic CA1 region of the rat brain. Fourfold immunofluorescence labeling microglia with Iba1 (green), astrocytes with GFAP (red), neurons with NeuN (yellow), and nuclei with DAPI (blue). SO - stratum oriens, SP – stratum pyramidale, SR – stratum radiatum, SLM – stratum lacunosum moleculare. The scale bar represents 50 μm. (A) Ctrl – control brain, (B) Isch – post-ischemic brain, (C) Quantification of the mean pixel intensities for Iba1 and GFAP signals of post-ischemic vs. control animals with 2 years survival. Values are presented as mean ± SEM. *** p<0.001. nCtrl = 16, nIsch = 17, n = number of analyzed cross sections. (D) Schematic representation of the rat hippocampus level with CA1 region indicated.