Figure 2. Effect of EA pretreatment on the dendritic spine density in cortical neurons in rats with CIR injury. (A) The Golgi-cox staining for the dendritic spine of neurons in the peri-ischemic cortex in the sham group (a), the I/R group (b), the I/R + EA group (c), and the I/R + EA + EX527 group (d) (100×). High magnification images are shown in the small windows (400×). The graphs below (a), (b), (c), and (d) were enlarged with 1000×, respectively. Scale bar, 100×: 200 μm, 400×: 100 μm; 1000×: 10 μm. (B) The dendritic spine density in cortical neurons. Data were presented as the mean ± SEM (n=3). **P<0.01 vs. sham group. ##P<0.01 vs. I/R group; &&P<0.01 vs. I/R + EA group.