Research Paper Volume 12, Issue 13 pp 13220—13233

Cancer-associated fibroblasts-derived gamma-glutamyltransferase 5 promotes tumor growth and drug resistance in lung adenocarcinoma

Figure 3. Targeting GGT5 in CAFs decreases the LUAD cell growth in vitro. (A) Gene Ontology Enrichment analysis (biological process) of GGT5 using Coexpedia internet tool (http://www.coexpedia.org/). (B) Conditioned media from CAFs transfected with scramble (Scramble-M) or siRNA targeting GGT5 (siGGT5-M) were used to treat LUAD cells A549 and LAC1, respectively, and cell growth curves were measured for six days. (C) Representative images of the foci formation of A549 cell in monolayer culture treated with Scramble-M or siGGT5-M. The number of foci was counted in right panel. (D) Representative images of the spheres formation in soft agar of A549 cell treated with Scramble-M or siGGT5-M. The number of spheres was analyzed in right panel. In panels B-D, data represent mean ± SEM. **, P < 0.01; ***, P < 0.001.