Research Paper Volume 12, Issue 13 pp 13234—13254

Increasing the expression of microRNA-126-5p in the temporal muscle can promote angiogenesis in the chronically ischemic brains of rats subjected to two-vessel occlusion plus encephalo-myo-synangiosis

Figure 15. Procedures used for establishment of the 2VO+EMS rat model. (A, B) Procedures used for CCA ligation (the black arrow indicates the left CCA). Representative images showing the EMS procedures, including (C) reflection of the skin and TM from the skull, (D) opening of the dura (the black asterisk indicates the TM tissue) and (E) stitching of the TM and DM with 10-0 Prolene (the black arrow indicates the DM). (F) Schematic drawing showing the EMS procedure and the four injection sites for the miR-126-5p agomir in the TM. (G) MRI-T2 film showing a lack of infarction after the 2VO procedures. (H) Representative graph of CBF changes before and after 2VO. (I) Doppler flowmetry results showing that the CBF values in 2VO rats (n = 8) decreased to 25.8 ± 2.6% of the baseline levels obtained for the 2VO sham group (n = 8). The error bars represent the ±SDs. EMS: encephalo-myo-synangiosis; TM: temporal muscle; DM: dura mater; CBF: cerebral blood flow; CCA: common carotid artery.