Research Paper Volume 12, Issue 13 pp 13234—13254

Increasing the expression of microRNA-126-5p in the temporal muscle can promote angiogenesis in the chronically ischemic brains of rats subjected to two-vessel occlusion plus encephalo-myo-synangiosis

Figure 9. Immunofluorescence results showing the level of CD31 and eNOS expression in the brain tissues from each group. (A) Dual immunofluorescence staining showing CD31(+) and eNOS(+) cells in the ischemic brains in each group. Bar = 20 μm. (B) Representative dual immunofluorescence staining showing both CD31(+) and eNOS(+) cells. Bar = 5 μm. The white arrow indicates a CD31(+) cell, and the yellow arrow indicates an eNOS(+) cell. (C) Counts of CD31(+), eNOS(+) and CD31/eNOS(+) cells in each group. The error bars represent the ±SDs. eNOS: endothelial nitric oxide synthase.