Research Paper Volume 12, Issue 13 pp 13255—13280

Circular RNA circMBOAT2 promotes prostate cancer progression via a miR-1271-5p/mTOR axis

Figure 1. Identification and characteristics of circMBOAT2 in PCa cells. (A) A heatmap showing circMBOAT2 ranks among the top 1% most enriched circRNAs in 144 cases of prostate cancer tissues (GSE113120). (B) qRT-PCR analysis of circMBOAT2 expression in PCa cell lines. (C) qRT-PCR analysis of circMBOAT2 abundance using oligo-dT primers and random primers in cDNA synthesis. (D) Gel electrophoresis for PCR products of circMBOAT2 and linear MBOAT2 derived from cDNA and gDNA. (E) The formation of circMBOT2, derived from exons 2 and 3 of the MBOAT2 gene. The back-splice junction of circMBOAT2 was confirmed by Sanger sequencing. (F, G) qRT-PCR analysis of the abundance of circMBOAT2 and MBOAT2 in PC-3 and DU145 cells treated with actinomycin D at the indicated times. (H) qRT-PCR analysis of the abundance of circMBOAT2 and MBOAT2 in PC-3 and DU145 cells treated with or without RNase R. (I) qRT-PCR analysis of circMBOAT2 abundance in the nuclear and cytoplasmic fractions of PC-3 and DU145 cells. (J) Fluorescence in situ hybridization (FISH) for cellular distribution of circMBOAT2 in PC-3 cells. Scale bar: 20 μm. Data are displayed as mean ± SD. *p < 0.05; **p < 0.01.