Figure 4. circMBOAT2 functions as a sponge for miR-1271-5p. (A) A schematic diagram showing potential target miRNAs of circMBOAT2. (B, C) RNA pull-down assay revealed that circMBOAT2 interacts with miR-1271-5p in PC-3 and DU145 cells (D) RNAalifold predicted the binding site of miR-1271-5p on circMBOAT2. (E) Wild-type (wt) and mutated (mut) sequences of the predicted binding site between circMBOAT2 and miR-1271-5p. (F) Dual-luciferase reporter assays revealing binding properties of circMBOAT2 and miR-1271-5p. Renilla luciferase (Rluc) activity was normalized to firefly luciferase activity. (G). qRT-PCR analysis of the abundance of circMBOAT2 captured by biotin-labeled microRNA probes. (H) FISH assay showed the colocalization of circMBOAT2 and miR-1271-5p. Data are displayed as mean ± SD. *p < 0.05; **p < 0.01.