Figure 8. circMBOAT2 is associated with PCa progression and responds to mTOR inhibitor treatment. (A) Image of the xenograft subcutaneous models of PC-3 cells stably transfected with vector or circMBOAT2 plasmid, treated with rapamycin or PBS. (B, C) Tumor volumes and weights of subcutaneous xenograft tumors. (D) qRT-PCR analysis of circMBOAT2 expression in a cohort of 50 PCa patient tissues paired with their respective NATs. (E, F) The Kaplan-Meier curves for DFS of PCa patients in cohort 1 and cohort2. Median circMBOAT2 expression levels were used as the cutoff value for patient stratification. (G) Proposed model of circMBOAT2-mediated PI3K/Akt activation in PCa tumorigenesis and metastasis. Data are displayed as mean ± SD. *p < 0.05; **p < 0.01.