Figure 2. Cell transfected successfully. (A) RT-qPCR assay showed that circRNA _ 100859 was up-regulated in Lovo, HT29, SW480, HCT116 cells, *P < 0.05, **P < 0.01 versus HIEC cells. (B) RT-qPCR assay showed that circRNA_100859 was increased in HCT116 cells, and decreased in Lovo cells. (C) RT-qPCR assay demonstrated that miR-217 expression levels were significantly increased in the mimic group, and significantly reduced in inhibitor group. *P < 0.05, **P < 0.01 versus corresponding NC group.