Research Paper Volume 12, Issue 13 pp 13338—13353

circRNA_100859 functions as an oncogene in colon cancer by sponging the miR-217-HIF-1α pathway

Figure 7. HIF-1α was directly targeted by miR-217. (A) The potential binding sequences of HIF-1α to miR-217. (B) T The relative luciferase activities were estimated after co-transfection with HIF-1α-wt or mut and miR-217 mimics or miR-217 NC. **P<0.01 vs. empty vector+NC. #P>0.05 vs. mut+mimic. (C) HIF-1αwas significantly over-expressed in colon cancer tissues (n=50), *P < 0.05, **P < 0.01 versus adjacent noncancer tissues. (D) Pearson's analysis suggested that the negative correlation between HIF-1α and miR-217. (E, F) RT-qPCR demonstrated that HIF-1α mRNA expression were significantly decreased in miR-217 mimic group, and dramatically increased in miR-217 inhibitor group. *P < 0.05, **P < 0.01 versus miR-217 NC group.