Figure 2. CBR-470-1 inhibits MPP+-induced oxidative injury in SH-SY5Y neuronal cells. SH-SY5Y neuronal cells were pre-treated for 2h with CBR-470-1 (“CBR”, 10 μM) or the vehicle control (“Veh”), followed by MPP+ (3 mM) stimulation, cells were further cultured for applied time periods, relative lipid peroxidation levels (A), single strand DNA contents (B) and mitochondrial depolarization(JC-1 green fluorescence intensity, (C) were tested, and then cell viability and death examined by CCK-8 (D) and medium LDH release (E) assays, respectively. Cell apoptosis was evaluated by the assays mentioned in the text (F–H).“Veh” stands for the vehicle control. “Mock” stands for MPP+ single treatment (no pretreatment).“Ctrl” stands for no MPP+ stimulation. Bars stand for mean ± standard deviation (SD, n=5). * P< 0.05 vs. “Ctrl” cells. #P< 0.05 vs. “Veh”-pretreated cells. Experiments in this figure were repeated four times, with the similar results obtained.