Figure 4. Effects of PHC on rI/R-induced lung inflammation in rats. (A) Twenty-four hours after rI/R treatment, lung sections from each investigational group (sham, PHC [1 mg/kg], rI/R, and rI/R + PHC [1 mg/kg]; n = 8) were obtained, sectioned, stained with H&E and assessed for apoptosis (original magnification 200×, 400×). A scale from 0 to 3 was used to calculate the mean lung injury score. (B) The relative apoptotic index (%) in the lungs was determined by TUNEL analysis. (C) The wet/dry weight ratios of the lungs were measured. (D) A BCA assay was used to determine the total protein level in BALF. Data are presented as the mean ± S.D. (n = 8, Scale bar: 50 μm). *P < 0.05, **P < 0.01 vs. the sham group. #P < 0.05, ##P < 0.01 vs. the rI/R group.