Figure 6. Effects of PHC on rI/R-induced lung inflammatory cell infiltration in rats. (A) A hemocytometer was used to count the numbers of total cells, neutrophils and macrophages as a measure of inflammatory cell infiltration. (B) ELISAs were used to measure the concentrations of pro-inflammatory molecules (IL-1β, IL-6 and TNF-α) in BALF. (C, D) Lung sections were collected 24 h after rI/R treatment, and total protein was obtained from lung homogenates. Western blotting was used to measure the levels of COX-2 and iNOS. GAPDH was used as an internal control. Data are presented as the mean ± S.D. (n = 8). *P < 0.05, **P < 0.01 vs. the sham group. #P < 0.05, ##P < 0.01 vs. the rI/R group.