Research Paper Volume 12, Issue 13 pp 13400—13421

Penehyclidine hydrochloride inhibits renal ischemia/reperfusion-induced acute lung injury by activating the Nrf2 pathway

Figure 7. Effects of PHC on rI/R-induced Nrf2/AMPK signaling in the lungs of rats. Lung sections were collected 24 h after rI/R treatment. Lung homogenization was used to obtain the total protein, nuclear and cytoplasmic extracts. Western blotting was used to measure protein levels. (A) The nuclear concentration of Nrf2 and the protein levels of Nrf2 target enzymes (NQO1, GCLC, GCLM and HO-1). (B) The levels of p-AMPK, p-GSK3β, p-Akt, AMPK, GSK3β and Akt. GAPDH was used as an internal control for cytoplasmic and total proteins, while histone H3.1 was used as an internal control for nuclear proteins. Data are presented as the mean ± S.D. (n = 8). *P < 0.05, **P < 0.01 vs. the sham group. #P < 0.05, ##P < 0.01 vs. the rI/R group.