Figure 8. Effects of PHC on rI/R-induced NF-κB and NLRP3 inflammasome signaling in the lungs of rats. Lung sections were collected 24 h after rI/R treatment. Lung homogenization was used to obtain the total protein, nuclear and cytoplasmic extracts. Western blotting was used to measure protein levels. (A) The levels of ASC, NLRP3, pro- and mature IL-1β, and caspase-1. (B) The nuclear concentration of p65 and the protein levels of IκBα and p-IκBα. GAPDH was used as an internal control for cytoplasmic and total proteins, while histone H3.1 was used as an internal control for nuclear proteins. Data are presented as the mean ± S.D. (n = 8). *P < 0.05, **P < 0.01 vs. the sham group. #P < 0.05, ##P < 0.01 vs. the rI/R group.