Figure 6. CPPs relieved Aβ-induced toxicity in rat primary neuron in vitro. On exposure to pre-aggregating Aβ1–42 or vehicle for 24h, rat primary neuron was treated with or without CPPs at the indicated concentrations of 100, 200 μg/ml for 24 h. (A) The expression of PSD95 and synaptotagmin (SYT) proteins in the primary neuron were detected by Western blotting, GAPDH as a loading control. (B, C) Quantitative analysis of the blots, n = 3. (D) Immunofluorescence staining was used to measure the expression of SYP in primary neuron. (E) Quantitative analysis of fluorescence intensity. Data were from 3 independent experiments. P value significance is calculated from a one-way ANOVA. **P < 0.01, ***P < 0.001 vs. control, #P < 0.05, ##P < 0.01vs. cells with Aβ treatment but without CPPs treatment, n = 3.