Research Paper Volume 12, Issue 13 pp 13463—13476

Mechanistic study of mtROS-JNK-SOD2 signaling in bupivacaine-induced neuron oxidative stress

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Figure 2.

Oxidative injury, JNK phosphorylation and SOD2 transcription were elevated in SH-SY5Y cells treated with BPV. (A) LDH in cells treated with different concentration (0.5, 2.0, 4.0 mM) of BPV for 60 min; (B) LDH in cells treated with 2.0 mM BPV for 30, 60, or 90 min.; (C, D) MDA and 8-OHdG production in cells treated with 2.0 mM BPV for 30, 60, or 90 min; (E, F) the fluorescence intensities of mtROS in cells incubation with 2.0 mM BPV for 30, 60, or 90 min; (G, H) the western blot analysis shows JNK and p-JNK in SH-SY5Y cells incubation with 2.0 mM BPV for 30, 60, or 90 min; (I, J) the western blot analysis showed SOD2 expression in cells incubation with 2.0 mM BPV for 30, 60, or 90 min. Values are the mean± SEM of n = 3. *: P< 0.05 compared with the group Con.