Research Paper Volume 12, Issue 13 pp 13477—13487

Hydrostatic pressure induces osteogenic differentiation of adipose-derived mesenchymal stem cells through increasing lncRNA-PAGBC

Figure 1. HP upregulates PAGBC and induces osteogenic differentiation of AMSCs. (A) Isolated human AMSCs were validated for MSC property (positive for CD73, CD90 and CD105, negative for CD34, CD45 and HLA-DR) by flow cytometry. (B) AMSCs in culture. (CE) AMSCs were cultured in osteogenic differentiation media under normal pressure (NP) culture versus HP culture. Osteogenic differentiation of AMSCs was determined by Von kossa staining, shown by quantification (C) and by representative images (D). (E) RT-qPCR for PAGBC. N=5. *p<0.05. Scale bars are 50μm.