Research Paper Volume 12, Issue 13 pp 13477—13487

Hydrostatic pressure induces osteogenic differentiation of adipose-derived mesenchymal stem cells through increasing lncRNA-PAGBC

Figure 2. PAGBC is a ceRNA for miR-133b in AMSCs. (A) MiR-133b was a specific PAGBC-targeting miRNA, analyzed by miRcode. (B) Plasmids that overexpress PAGBC or deplete PAGBC were prepared. Plasmids carrying a scramble sequence were used as controls. These plasmids were used to transfect AMSCs and RT-qPCR for PAGBC was assessed in these cells. (C, D) We prepared luciferase reporter for wildtype (wt) miR-133b and miR-133b with a mutant at the PAGBC binding site (mut). The luciferase reporter assay was performed on AMSCs, using either miR-133b wt (C) or miR-133b mut (D). N=5. *p<0.05. NS: non-significant.