Research Paper Volume 12, Issue 13 pp 13477—13487

Hydrostatic pressure induces osteogenic differentiation of adipose-derived mesenchymal stem cells through increasing lncRNA-PAGBC

Figure 3. HP induces osteogenic differentiation of AMSCs through PAGBC. AMSCs transfected with scr were kept in either NP or HP, while AMSCs transfected with PAGBC were kept in NP, AMSCs transfected with shPAGBC were kept in HP, to compare with AMSCs in NP and AMSCs in HP, respectively. (A, B) RT-qPCR for PAGBC (A) and miR-133b (B) in AMSCs. (C, D) Osteogenic differentiation of AMSCs was determined by Von kossa staining, shown by quantification (C) and by representative images (D). (E) RT-qPCR for osteogenesis-associated genes, collagen I (Col I) and osteopontin (OPN). N=5. *p<0.05. Scale bars are 50μm.