Research Paper Volume 12, Issue 13 pp 13477—13487

Hydrostatic pressure induces osteogenic differentiation of adipose-derived mesenchymal stem cells through increasing lncRNA-PAGBC

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Figure 5.

miR-133b suppresses osteogenic differentiation of AMSCs through RUNX2. (A, B) Plasmids that overexpress or deplete RUNX2 were validated by RT-qPCR (A) and by ELISA (B) for RUNX2. (CG) AMSCs were transfected with either miR-133b or as-miR-133b, compared to null controls. In another two groups, AMSCs were co-transfected with miR-133b and RUNX2 or with as-miR-133b and shRUNX2. (C, D) RT-qPCR (C) and ELISA (D) for RUNX2. (E, F) Osteogenic differentiation of AMSCs was determined by Von kossa staining, shown by representative images (E) and by quantification (F). (G) RT-qPCR for osteogenesis-associated genes, collagen I (Col I) and osteopontin (OPN). N=5. *p<0.05. Scale bars are 50μm.