Research Paper Volume 12, Issue 13 pp 13594—13617

The transcription factor CREB acts as an important regulator mediating oxidative stress-induced apoptosis by suppressing αB-crystallin expression

Figure 8. ChIP assays to demonstrate that CREB binds to the promoter of αB-crystallin gene in vivo. qChIP experiments revealed that in the in vivo condition, CREB displayed stronger affinity with M10 site (B), suggesting that CREB may interact with its partner to bind to the M10 site, and to a less degree, to the M8 site (A). All experiments were repeated three times. Error bar represents standard deviation, N=3. * p<0.05; ** p<0.01; *** p<0.005; NS, statistically not significant.