Figure 1. Hic-5 was up-regulation in human osteosarcoma tissues and cell lines. (A) RT-PCR was employed to detect the expression of Hic-5 in tumor and normal tissues. n=15, **P<0.01. (B and C) The mRNA and protein expression of Hic-5 was measured in MG-63, HOS, U2-OS and SAOS-2 cells, hFOB1.19 cells were described as control. n=8, *P<0.05. (D) Hic-5 staining in slides from osteosarcoma (high grade and low grade) and adjacent normal bone tissues.