Research Paper Advance Articles pp 23598—23608

Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway

Figure 5. Exosomal-Hic-5 regulated the development of OS via inhibiting Wnt/β-catenin signal pathway. (A) CoIP assay was performed to confirm the relationship between Hic-5 and smad4. (B) The expression of Wnt/β-catenin signal pathway elements (sFRP1, Smad4, Wnt1and β-catenin) were detected in MG-63 cells after RFP-shRNA transfection. n=6, *P<0.05. (C) The expression of sFRP1, Smad4, Wnt1, β-catenin were detected in tumor from treated-MG-63 cell injection mice. n=10, *P<0.05. (D) The expression of Wnt/β-catenin signal pathway elements (sFRP1, Smad4, Wnt1and β-catenin) were detected in MG-63 cells after exosomes co-cultured. n=6, *P<0.05. (E) The expression of sFRP1, Smad4, Wnt1, β-catenin were detected in tumor from exosomes co-cultured-MG-63 cell injection mice. n=10, *P<0.05. (F) Wnt pathway activation by treatment with RFP-sh-Hic5/RFP-sh-NC regulates ß-Catenin signaling mediated TCF/LEF reporter activity. n=3, *P<0.05. (G) Subcellular localization of active β-catenin and total β-catenin in MG-63 cells by immunofluorescence staining.