Research Paper Volume 12, Issue 14 pp 14830—14848

FGF2-induced PI3K/Akt signaling evokes greater proliferation and adipogenic differentiation of human adipose stem cells from breast than from abdomen or thigh

Figure 2. Different proliferation and differentiation abilities of haASCs, htASCs and hbASCs in vitro. (A) Cell proliferation as assessed by a CCK-8 assay. The proliferation rate and growth capacity of htASCs were similar to those of haASCs or hbASCs, but there was no significant difference in cell doubling time among htASCs, haASCs, and hbASCs. (B) Colony formation assays. Cells within the colonies exhibited long-spindle, short-spindle, short-round, or long-narrow morphology. After 14 days, colony forming units (CFUs) were significantly higher for hbASCs than for htASCs or haASCs (P < 0.05). (C) Adipogenic differentiation assay of htASCs, haASCs, and hbASCs in vitro. Adipocyte density and lipid concentration were significantly different between hbASCs and the other two ASC types. *P<0.05, **P<0.05