Research Paper Volume 12, Issue 14 pp 14830—14848

FGF2-induced PI3K/Akt signaling evokes greater proliferation and adipogenic differentiation of human adipose stem cells from breast than from abdomen or thigh

Figure 4. Genes differentially expressed between haASCs and hbASCs. (A) Volcano map. Red means up-regulated genes; blue means down-regulated genes. (B) Circplot associated with paracrine genes of ASCs. (C) ELISA of paracrine factors produced by htASCs, haASCs, and hbASCs in vitro. Higher levels of FGF2, TIMP-1, and SDF-1 were secreted by hbASCs than by htASCs or haASCs at 3 and 7 d. *P<0.05. (D) Differences in expression of genes related to paracrine function and adipogenesis across the three types of transplants. The hbASC tissue showed up-regulation of TIMP-1, CXCR4, FGF2, FGF9, PPAγ2, FAD24 and ADD1, but down-regulation of FGF3 and FGF6 relative to htASC and haASC tissue. C/EBPα expression was similar between htASC and haASC tissue. *P < 0.05, **P < 0.05, #P < 0.05