Research Paper Volume 12, Issue 14 pp 15021—15036

NVD-BM-mediated genetic biosensor triggers accumulation of 7-dehydrocholesterol and inhibits melanoma via Akt1/NF-ĸB signaling

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Figure 6.

Schematic of a genetic circuit in melanoma. (A) A gene circuit, with four modules was activated by two up-regulated transcription factors (RELA and STAT1), to express cholesterol 7-desaturase NVD-BM, and directly catalyzing cholesterol to 7-DHC, to repress melanoma growth and migration. (B) Gene circuit diagram constructed in melanoma cells based on CRISPR/Cas9 and Tet-ON systems.