Research Paper Volume 12, Issue 14 pp 15021—15036

NVD-BM-mediated genetic biosensor triggers accumulation of 7-dehydrocholesterol and inhibits melanoma via Akt1/NF-ĸB signaling

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Figure 7.

The activated genetic circuit inhibits melanoma cell growth and migration. (A) A375 and A2058 cells were co-transfected with genetic circuit plasmids. The relative expression of the reporter gene NVD-BM compared to GAPDH, was quantified by qRT-PCR. (B) A375 and A2058 cell proliferation was assessed after treatment with and without doxycycline for 48 h. (C, D) The migration rates of 6×104 A375 cells transfected with genetic circuit plasmids (± doxycycline) were assessed over 36 h by RTCA assay. (***p < 0.001).