Research Paper Volume 12, Issue 13 pp 13762—13790

Aging affects sex- and organ-specific trace element profiles in mice

Figure 6. Activity and expression levels of Nrf2 target genes. The enzyme activities of the Nrf2 targets NQO1 (A) and total GST (C) were determined by activity assays, whereas the relative protein levels of FTH (B) normalized to the house-keeping gene GAPDH were analyzed by Western blot in liver tissue of adult (24 weeks) and old (109-114 weeks) male and female mice (n = 4-5). Statistical testing based on Two-Way ANOVA and post hoc analysis using Bonferroni’s test with ** p < 0.01 vs. adult and # p < 0.05, ### p < 0.001 vs. male.