Research Paper Volume 12, Issue 15 pp 15566—15580

The association between KLF4 as a tumor suppressor and the prognosis of hepatocellular carcinoma after curative resection


Figure 5. KLF4 inhibits the invasion of HCC cells. (A) Transwell assay was determined to detect the effect of KLF4 on cell invasion in PLC/PRF/5 and SMMC-7721 cells. Student’s t-test was used in bar charts. (B) Changes in cell morphology in SMMC-7721 with shKLF4. (C) Western blot and qPCR were used to detect EMT-associated protein expression in steadily transfected PLC/PRF/5 and SMMC-7721 cells. P < 0.05 was considered statistically significant. * P < 0.05, ** P < 0.01, *** P < 0.001.