KLF4 inhibits the invasion of HCC cells. (A) Transwell assay was determined to detect the effect of KLF4 on cell invasion in PLC/PRF/5 and SMMC-7721 cells. Student’s t-test was used in bar charts. (B) Changes in cell morphology in SMMC-7721 with shKLF4. (C) Western blot and qPCR were used to detect EMT-associated protein expression in steadily transfected PLC/PRF/5 and SMMC-7721 cells. P < 0.05 was considered statistically significant. * P < 0.05, ** P < 0.01, *** P < 0.001.