Research Paper Volume 12, Issue 21 pp 21114—21128

Hsa_circ_0043278 functions as competitive endogenous RNA to enhance glioblastoma multiforme progression by sponging miR-638

Figure 1. Expression and verification of circ_0043278 in glioma cells and tissues. qRT-PCR results for (A) circ_0043278 expression in glioma tumor tissues and their paired adjacent non-tumor tissues. Results represent the mean±SD for three independent experiments (N=30, **p<0.01, as determined by paired Student’s t-test); (B) circ_0043278 expression in glioma tissues of different grades. Results represent the mean±SD for three independent experiments (N=15 (I-II), N=15 (III-IV), *p<0.05, as determined by unpaired Student’s t-test); (C) circ_0043278 expression in four glioma cell lines and normal human astrocytes (NHAs). Results represent the mean±SD for three independent experiments (*p<0.05, **p<0.01, as determined by one-way ANOVA with Dunnett’s multiple comparisons test); (D) circ_0043278 expression after stable transfection of circ_0043278-shRNA1#/2#, or negative control (NC). Results represent the mean±SD for three independent experiments (*p<0.05, **p<0.01, as determined by one-way ANOVA with Dunnett’s multiple comparisons test); CCK-8 assay results demonstrated that (E, F) shRNA-mediated circ_0043278 silencing suppresses U87 and U251 cell growth. Results represent the mean±SD of three independent experiments (*p<0.05, **Pp<0.01, as determined by one-way ANOVA with Dunnett’s multiple comparison test); FISH results showed that (G) circ_0043278 was predominantly localized in the cytoplasm. Nuclei were stained with DAPI and circ_0043278 probes were labeled with Alexa Fluor 555 (scale bar = 50 μm in all panels).