Research Paper Volume 12, Issue 21 pp 21114—21128

Hsa_circ_0043278 functions as competitive endogenous RNA to enhance glioblastoma multiforme progression by sponging miR-638

Figure 7. Circ_0043278/MiR-638 regulates HOXA9/Wnt/β-catenin signaling pathway expression. (AD) U87 and U251 cells were stably transfected with NC or cic_0043278-shRNA1# or co-transfected with cic_0043278-shRNA1# and miR-638 inhibitor. HOXA9 and Wnt/β-catenin signaling protein expression were evaluated using western blotting. Results represent the mean±SD for three independent experiments (*p<0.05, **p<0.01, as determined by one-way ANOVA with Dunnett’s multiple comparisons test).