Research Paper Volume 12, Issue 21 pp 21114—21128

Hsa_circ_0043278 functions as competitive endogenous RNA to enhance glioblastoma multiforme progression by sponging miR-638

Figure 8. Circ_0043278 acts as miR-638 sponge to enhance tumorigenesis invivo. (A) Images of mouse tumors one month after implantation with U87 cells stably transfected with NC or cic_0043278-shRNA1# or co-transfected with cic_0043278-shRNA1# and LV-anti-miR-638 subcutaneously. Tumors were dissected and photographed after one month; (B) Tumor weight was calculated after euthanasia (N = 6 in each group, *p<0.05, **p<0.01, as determined by one-way ANOVA with Bonferroni’s multiple comparisons test); (C) Tumor volumes were recorded every six days after mice were injected with stable glioma cells (N = 6 in each group, **p<0.01, ***p<0.001, *** p<0.0001, as determined by two-way ANOVA with Dunnett’s multiple comparisons test); (D, E) Western blotting was used to detect HOXA9 protein levels and Wnt/β-catenin signaling. Results represent the mean±SD for three independent experiments (*p<0.05, **p<0.01, as determined by one-way ANOVA with Dunnett’s multiple comparisons test).