Figure 5. Clonogenic and differentiation properties of MSCs. (A) The pictures show representative crystal violet staining (CFU-assay) of clones obtained after 14 days of incubation, with MSCs obtained from obese and control animals. The mean number of clones per 1,000 cells plated in 100 mm dish (± SD, n = 6) is indicated in the graph below. (B) Adipocyte, osteocyte, and chondrocyte differentiation of MSCs obtained from obese and control animals. The figure shows representative images of Oil Red Oil (adipocytes), Alizarin Red S (osteocytes), and Safranin O (chondrocytes) staining for every experimental condition. (C) Qualitative RT-PCR analysis of differentiation markers expressed in proliferating MSC cultures from obese and normal samples. The picture shows a representative gel-electrophoresis analysis.