Research Paper Volume 12, Issue 13 pp 12648—12668

Lack of retinoid acid receptor-related orphan receptor alpha accelerates and melatonin supplementation prevents testicular aging

Figure 6. Enzyme histochemistry in the young and aged mice. (A, D) In both control and melatonin groups, ATPase activities increased in Leydig cells (arrow) and active germ cells (arrowheads). (B) ATPase activities were concentrated in spermatogonia and spermatocytes (arrowheads) in young mutant mice. (C) In the aged mice, the staining reaction increased in interstitial cells (arrow) and some spermatogonia (arrowheads). (E) In control, alkaline phosphatase staining was detected in the elongated spermatids (arrowhead). (F) In young mutant mice, the reaction was intense in the germ cells (arrowhead) and Sertoli cells and their associated spermatids (arrows). (G) In aged mutant mice, the activity increased in spermatogonia and spermatocytes (arrowhead) and the Leydig cells showed high acid phosphatase activity (arrow). (H) In the melatonin group, alkaline phosphatase activity was detected in the active Sertoli cells (arrowheads) and Leydig cells (arrows).