Research Paper Volume 12, Issue 22 pp 22538—22549

Inflammatory factors and amyloid β-induced microglial polarization promote inflammatory crosstalk with astrocytes

Figure 4. Various cytokines were secreted by different astrocyte groups. (AE) Astrocytes in different groups were stained with antibodies against cytokines. (A1A6) Staining for IL-1β (Red). (B1B6) TNF-α (Green). (C1C6) IL-4 (Red). (D1D6) IL-6 (Green). (E1E6) IL-10 (Red). Blue represents DAPI staining. White arrows denote IL-1β+ cells. Pink arrows denote TNF-α+ cells. Yellow arrows denote IL-4+ cells. Beige arrows denote IL-6+ cells. Blue arrows denote IL-10+ cells. (FJ) The numbers of positive cells in different groups (n=3). (KO) Quantitative data on the relative cytokine levels in astrocytes (n=3). Error bars, S.E.M. Compared with AS-Con, *p<0.05, **p<0.01. Compared with AS-LPS/IFN-γ, #p<0.05, ##p<0.01. Compared with AS-IL-4/IL-13, ○p<0.05, ○○p<0.01. Compared with AS-Aβ, Δp<0.05, ΔΔp<0.01. Compared with AS-LPS/IFN-γ+Aβ, ↓p<0.05, ↓↓p<0.01. One-way ANOVA was performed with Tukey’s correction.