Figure 4. Various cytokines were secreted by different astrocyte groups. (A–E) Astrocytes in different groups were stained with antibodies against cytokines. (A1–A6) Staining for IL-1β (Red). (B1–B6) TNF-α (Green). (C1–C6) IL-4 (Red). (D1–D6) IL-6 (Green). (E1–E6) IL-10 (Red). Blue represents DAPI staining. White arrows denote IL-1β+ cells. Pink arrows denote TNF-α+ cells. Yellow arrows denote IL-4+ cells. Beige arrows denote IL-6+ cells. Blue arrows denote IL-10+ cells. (F–J) The numbers of positive cells in different groups (n=3). (K–O) Quantitative data on the relative cytokine levels in astrocytes (n=3). Error bars, S.E.M. Compared with AS-Con, *p<0.05, **p<0.01. Compared with AS-LPS/IFN-γ, #p<0.05, ##p<0.01. Compared with AS-IL-4/IL-13, ○p<0.05, ○○p<0.01. Compared with AS-Aβ, Δp<0.05, ΔΔp<0.01. Compared with AS-LPS/IFN-γ+Aβ, ↓p<0.05, ↓↓p<0.01. One-way ANOVA was performed with Tukey’s correction.