Figure 5. Expression of p65 and Stat6 in different groups of microglia and astrocytes. (A, B) p65 and Stat6 staining in microglia. (A1–A6) Double-staining for p65 and Iba1. (B1–B6) Stat6 and Iba1. Green represents Iba1+ cells, red represents p65+ or Stat6+ cells and blue represents DAPI staining. White arrows denote p65+Iba1+ cells. Pink arrows denote Stat6+Iba1+ cells. (C, D) p65 and Stat6 staining in astrocytes. (C1–C6) p65+ cells (Red and yellow arrows). (D1–D6) Stat6+ cells (Green and Beige arrows). (E–H) The numbers of positive cells in different groups (n=3). (I–L) Quantitative data on the relative levels of p65 or Stat6 (n=3). Error bars, S.E.M. Compared with Con or AS-Con, *p<0.05, **p<0.01. Compared with LPS/IFN-γ or AS-LPS/IFN-γ, #p<0.05, ##p<0.01. Compared with IL-4/IL-13 or AS-IL-4/IL-13, ○p<0.05, ○○p<0.01. Compared with Aβ or AS-Aβ, Δp<0.05, ΔΔp<0.01. Compared with LPS/IFN-γ+Aβ or AS-LPS/IFN-γ+Aβ, ↓p<0.05, ↓↓p<0.01. One-way ANOVA was performed with Tukey’s correction.