Research Paper Volume 12, Issue 22 pp 22538—22549

Inflammatory factors and amyloid β-induced microglial polarization promote inflammatory crosstalk with astrocytes

Figure 5. Expression of p65 and Stat6 in different groups of microglia and astrocytes. (A, B) p65 and Stat6 staining in microglia. (A1A6) Double-staining for p65 and Iba1. (B1B6) Stat6 and Iba1. Green represents Iba1+ cells, red represents p65+ or Stat6+ cells and blue represents DAPI staining. White arrows denote p65+Iba1+ cells. Pink arrows denote Stat6+Iba1+ cells. (C, D) p65 and Stat6 staining in astrocytes. (C1C6) p65+ cells (Red and yellow arrows). (D1D6) Stat6+ cells (Green and Beige arrows). (EH) The numbers of positive cells in different groups (n=3). (IL) Quantitative data on the relative levels of p65 or Stat6 (n=3). Error bars, S.E.M. Compared with Con or AS-Con, *p<0.05, **p<0.01. Compared with LPS/IFN-γ or AS-LPS/IFN-γ, #p<0.05, ##p<0.01. Compared with IL-4/IL-13 or AS-IL-4/IL-13, ○p<0.05, ○○p<0.01. Compared with Aβ or AS-Aβ, Δp<0.05, ΔΔp<0.01. Compared with LPS/IFN-γ+Aβ or AS-LPS/IFN-γ+Aβ, ↓p<0.05, ↓↓p<0.01. One-way ANOVA was performed with Tukey’s correction.