Research Paper Volume 12, Issue 20 pp 20139—20151

Circular RNA circ_PVT1 induces epithelial-mesenchymal transition to promote metastasis of cervical cancer

Figure 3. Upregulation of circ_PVT1 induces migration and invasion of CC cells by regulating EMT. (A) The transfection efficiency was verified by RT-PCR. n=6, **P<0.01. (B) The CCK8 assay was performed in cervical cancer cell lines. n=8, *P < 0.05. (C) Clone formation assays in cervical cancer cell lines. (D) Wound healing assay was carried out for migration. (E, F) The migration and invasion were explored by transwell. n=6, *P<0.05. (G) The number of nodules was statistics for detecting lung metastasis. n=6, *P<0.05. (H) The expression of metastasis-associated protein, E-cadherin, Vimentin, N-cadherin and SNAIL were detected. n=6, *P<0.05. (I) Immunofluorescence representative images of E-cadherin, Vimentin, N-cadherin. n=6, *P<0.05, **P<0.01.