Figure 4. Circ_PVT1 binds directly to miR-1286 in C33A cells. (A) Schematic for predicting miR-1286 binding sites of circ_PVT1 (https://circinteractome.nia.nih.gov/api/v2/mirnasearch?circular_rna_query=hsa_circ_0009143&mirna_query=hsa-miR-1286&submit=miRNA+Target+Search). (B) The luciferase assay was performed to verify the interaction between miR-1286 and circ_PVT1. n=5, *P<0.05. (C) Binding situation between circ_PVT1 and miR-1286 verified using RNA pull-down. (D) The enrichment of circ_PVT1 was detected by RIP assay. n=5, **P<0.01. (E) Co-localization between circ_PVT1 and miR-1286 was revealed by fluorescence in situ hybridization. (F) The expression of miR-1286 were assessed in C33A cells. n=10, **P<0.01.